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How to Reconstitute Lyophilised Peptides
The general laboratory workflow for reconstituting a freeze-dried peptide, and the factors that affect solubility.
Lyophilised peptides ship as a dry powder for stability. Before use in the laboratory they are dissolved in an appropriate solvent, a step known as reconstitution. The right approach depends on the peptide's chemistry, so always work from your own protocol and the product documentation.
Choosing a solvent
Sterile or bacteriostatic water is a common starting point for many water-soluble peptides. Hydrophobic or aggregation-prone sequences may need a small amount of a co-solvent first. Because solubility varies widely, test a small aliquot before committing the whole vial.
General workflow
Let the sealed vial reach room temperature before opening to avoid condensation. Add solvent slowly down the side of the vial rather than directly onto the powder, then swirl gently. Avoid vigorous shaking, which can denature sensitive peptides. Give the material time to dissolve fully before use.
After reconstitution
A reconstituted peptide is generally less stable than the dry powder. Aliquot to avoid repeated freeze-thaw cycles and store per your protocol. Record the concentration and date on each aliquot for traceability.